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. 2021 Aug 11;11:158. doi: 10.1186/s13578-021-00670-w

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — The effect of PirB knockdown in the retina by AAV PirB shRNA. A GFP (green, 488) expression in the retina transfected with AAV PirB shRNA or AAV shRNA for 21 days. red * represents the injection site, and the purple dotted line shows the area without the retinal tissue. Scale bars: 150 μm. B GFP staining in the PBS group. DAPI represents nuclear staining (blue fluorescence). Scale bar: 250 μm. C GFP (green, 488) and β-Ш Tubulin (red, 594) fluorescent staining in the AAV PirB shRNA group. DAPI represents nuclear staining (blue). Scale bar: 250 μm. Small white arrows in c show GFP and β-Ш Tubulin co-localization. D GFP (green, 488) and Vimentin (red, 594) fluorescent staining in the AAV PirB shRNA group. DAPI represents nuclear staining (blue). Scale bar: 250 μm. Small white arrows in d show GFP and Vimentin co-localization. E Bar charts showing PirB expression in the retina tissue. GAPDH was used as an internal reference. The ratios of gray values of target proteins to those of respective internal references are provided. The experiment was repeated three times (n = 4). Data represent the mean ± standard deviation. One-way ANOVA was performed, and Student’s t-test was used for group-pair comparisons used. **, p < 0.01