Table 1.
List of primary and secondary antibodies used for western blotting and immunohistochemistry (IHC)
| Antibody | Source | Lot/Cat No. | Dilution | |
|---|---|---|---|---|
| Primary antibodies | WB | IHC | ||
| Goat anti-PirB | R&D, Minnesota, USA | AF2754 | 1:1000 | 1:100 |
| Rabbit anti-Vimentin | Abcam, Cambridge, UK | ab92547 | 1:500 | |
| Rabbit anti-Phospho Stat3 Y705 | Abcam, Cambridge, UK | ab76315 | 1:5000 | |
| Rabbit anti-Stat3 | Abcam, Cambridge, UK | Ab68153 | 1:2000 | |
| Rabbit anti-GFP | Abcam, Cambridge, UK | Ab290 | 1:200 | |
| Rabbit anti-GAP43 | Cell Signaling, Boston, USA | #8945 | 1:200 | |
| Rabbit anti-CNTF | Abcam, Cambridge, UK | ab175387 | 1:1000 | |
| Rabbit anti-beta III Tubulin | Abcam, Cambridge, UK | ab18207 | 1:500 | |
| Mouse anti-beta III Tubulin | Abcam, Cambridge, UK | ab78078 | 1:500 | |
| Mouse anti-GAPDH | Abcam, Cambridge, UK | ab8245 | 1:5000 | |
| Secondary antibodies | ||||
| HRP-labeled anti-mouse IgG | Invitrogen, Poole, UK | G-21040 | 1:10,000 | |
| HRP-labeled anti-rabbit IgG | Invitrogen, Poole, UK | 31,460 | 1:10,000 | |
| HRP-labeled anti-Donkey IgG | R&D, Minnesota, USA | HAF109 | 1:1000 | |
| Alexa488 anti-goat IgG | Invitrogen, Poole, UK | A32814 | 1:300 | |
| Alexa594 anti-rabbit IgG | Invitrogen, Poole, UK | A32740 | 1:300 | |