Table 1.
qPCR primer sequences
| Marker | Gene | F/R | Primer (5′ to >3′) |
|---|---|---|---|
| Tenogenic | Tenomodulin (Tnmd) | F | GCGATAATGTGACCATGTACTG |
| R | GTCTTCTCCACCTTCACTTGC | ||
| Scleraxis (Scx) | F | GTTGAGCAAAGACCGTGACAG | |
| R | CCGTGACTCTTCAGTGGCAT | ||
| Tenascin C (Tnc) | F | TATCTGGTGCTGAACGGACTG | |
| R | CGGTTCAGCTTCTGTGGTAG | ||
| Tendon ECM | Collagen I, α1 (Col1a1) | F | AGCACGTCTGGTTTGGAGAG |
| R | GACATTAGGCGCAGGAAGGT | ||
| Collagen III, α1 (Col3a1) | F | CACGTAAGCACTGGTGGACA | |
| R | AGAAGTCTGAGGAATGCCAGC | ||
| Decorin (Dcn) | F | GAGGGAACTCCACTTGGACAAC | |
| R | CCAGCTCGGCAGAAGTCATT | ||
| Myogenic | α‐Smooth muscle actin (Acta2) | F | GTGATCACCATTGGAAACGAAC |
| R | GCATAGAGATCCTTCCTGATGTC | ||
| Fibrogenic | Connective tissue growth factor (Ctgf) | F | CAAGGACCGCACAGCAGTTG |
| R | AGAACAGGCGCTCCACTCTG | ||
| Osteogenic | Alkaline phosphatase (Alpl) | F | GCAATGAGGTCACATCCATC |
| R | CTCTGGTGGCATCTCGTTATC | ||
| Adipogenic | Fatty acid‐binding protein 4 (Fabp4) | F | GATGAAATCACCGCAGACGAC |
| R | CCAGCTTGTCACCATCTCGTT | ||
| Housekeeping gene | Ribosomal protein L4 (Rpl4) | F | CTTCGCCAGGCCAGAAATCA |
| R | TCTCGGATTTGGTTGCCAGTG | ||
| Ribosomal protein S29 (Rps29) | F | CACGGTCTGATCCGCAAATACG | |
| R | GCATGATCGGTTCCACTTGGTA |
Abbreviations: ECM, extracellular matrix; F, forward; qPCR, quantitative polymerase chain reaction; R, reverse.