TABLE 1.

Strains and plasmids used in this study

Strain or plasmid	Description	Reference
Strains
MGAS2221	Invasive clinical isolate, reference serotype M1	Sumby et al. (2006)
2221ΔccpA	MGAS2221 ΔccpA::spc	Shelburne et al. (2010)
2221‐CcpA‐V301A	MGAS2221 with V301A change in CcpA	This study
BL21‐pET‐His2‐CcpA	E. coli producing recombinant wild type GAS CcpA	Shelburne et al. (2008)
BL21‐pET‐His2‐V301A	E. coli producing recombinant GAS CcpA with V301A change	This study
BL21‐pET‐His2‐HPr	E. coli producing recombinant GAS HPr	Shelburne et al. (2010)
BL21‐pET21a‐HPrKP	E. coli producing recombinant GAS HPrK/P	Shelburne et al. (2010)
Plasmids
pET‐His2‐CcpA	pET‐His2 plasmid with GAS ccpA gene	Shelburne et al. (2008)
pET‐His2‐V301A	pET‐His2 plasmid with GAS ccpA gene encoding V301A change	This study
pET‐His2‐HPr	pET‐His2 plasmid with GAS HPr gene	Shelburne et al. (2010)
pET21a‐HPrK/P	pET21a plasmid with GAS HPrK/P gene	Shelburne et al. (2010)
pBBL740 ccpAV301A	pBBL740 plasmid with GAS ccpA gene encoding V301A change	This study