Figure 2.

Glycogen synthase kinase‐3β (GSK3β) inhibition by SB216763 decreased acetylcholinesterase (AChE) activity in primary cultured cortical neurons. (a) Over‐expression of GSK3β and tau in primary cortical neurons (GSK3β + tau) resulted in increased P‐tau levels, leading to an increase in AChE protein (blots probed with the N‐19 antibody) and activity, expressed relative to the controls transfected with the empty pCI vector. (b) Neurons transfected with GSK3β + tau were treated for 24 hr with the GSK3β inhibitor SB216763 or the vehicle alone (DMSO; control). A decrease was observed in the AChE protein (blots probed with the N‐19 antibody) and in AChE activity relative to the controls. Mean values ± SEM are represented of at least of 18 independent measurements from three independent experiments: *Significantly different from the control group (p <.05), as assessed by the Student's t test