Figure 5.

Acetylcholinesterase (AChE) colocalizes with phosphorylated tau (P‐tau) in cytoplasmatic regions in Chinese hamster ovary (CHO) cells. CHO cells that stably over‐express ACHE‐T variant were transfected with plasmid cDNAs that encode glycogen synthase kinase‐3β and tau to increase P‐tau levels. Immunoassay was performed and confocal images were obtained with a x63 oil immersive objective lens. From left to right we observe: stained nuclei with Hoechst in blue; AChE probed with anti‐N‐terminus AChE antibody plus anti‐rabbit IgG Alexa Fluor 488 in green; P‐tau with anti‐PHF‐tau antibody combined with an anti‐mouse IgG Cy5 in red; Merge image showing three channels overlay with a colocalization of P‐tau and AChE image insert on the left‐bottom showing pixels positive for both P‐tau and AChE marked in white. Representative images of n = 3 experiments are showed (scale bar = 5 µm)