Table 2.
Qualitative descriptions for each RNA extraction kit used.
| Qualitative table for extraction methods | New England Biolabs Monarch RNA MiniPrep | Qiagen AllPrep PowerViral DNA/RNA Kit | Zymo Quick-RNA Fecal/Soil Microbe MicroPrep | Zymo Quick-RNA Viral with Inhibitor Removal |
|---|---|---|---|---|
| Time of kit | 30 min | 1 h | 1.5 h | 20 min + 20 min for inhibitor removal |
| Price (at time of publication) | $17.9/sample | $24.47/sample | $10.44/sample | $24.23/sample |
| Extra equipment | Floor centrifuge | Floor centrifuge | Floor centrifuge | |
| Consumables | AMICON Ultrafilters | AMICON Ultrafilters | Nuclease Free Tubes (2 mL) | AMICON Ultrafilters; Nuclease Free Tubes (2 mL) |
| Type of wastewater concentrate | Filtrate | Filtrate | Pellet | Filtrate |
| Extra reagents | 100% Ethanol | B-Mercaptoethanol | 100% Ethanol | 100% Ethanol |
| Extra supplies | RNAse-free tubes | No | RNAse-free Tubes | RNAse-free tubes |
| Effectiveness | Lower | Lower | Higher | Higher |
| Overall turn-around Time for 4 Samples | 9 h | 9.5 h | 5 h | 9 h |
| Qualitative thoughts | If using this kit, we would suggest also using the RNA Cleanup Kit recommended by representatives from New England Biolabs | Lost a significant amount of RNA sample compared to other kits | Kit is tedious with larger sample volumes, but overall gave best results | Especially with Inhibitor Removal step, could provide a comparative alternative for Fecal Kit, but longer processing times |