Fig. 21.

Effects of FL118 on the modulation of potential molecular targets in the ccRCC cell lines UOK161, UOK111) and the Type 2 pRCC cell line, FHpRCC UOK262: Sub-confluent cells grown in complete medium in 6-well plates were treated with and without FL118 at the concentration of 100 nM for 16 h, 24 h and 48 h (A, B) or for 16 h at the concentration of 10 nM, 100 nM and 500 nM (C) as shown. Cells were then lysed for Western blot analyses with antibodies for the corresponding molecular protein targets. The expression of β-actin and GAPDH are the internal protein loading control as shown. Of note, HIF1α, MDM2 and Mdm4 were not detected in both UOK161 and UOK111 cells