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. 2021 Jul 29;9:692617. doi: 10.3389/fcell.2021.692617

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Copyright © 2021 Perin, Rossetti, Ricci, Cossellu, Lazzarini, Bethge, Voigt, Helmchen, Batti, Gantar and Pizzala.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Hindbrain slices from light-sheet 3D dataset of the whole-mount brain-bone preparation showing the extent and association of the CP. (A) Coronal slice showing CP attachment (highlighted in red) at the nodulus and paraflocculus. Scale bar: 2 mm. (B) Sagittal slice (different animal from A) showing the CSS with overlying tela coroidea (yellow line) and attachment to area postrema (asterisk). Scale bar: 1 mm. (C) Coronal slice showing the MHS (highlighted in red). Same animal as in (A). Scale bar: 2 mm. (D) Horizontal slice detail (reslice of stack in A,C) showing the dorsal cochlear nucleus (DCN) and flocculus (FL). Scale bar: 1 mm. (E) Coronal slice detail from animal in (A) showing the foramen of Luschka (arrow) with the associated CP. Scale bar: 1 mm. All sections were stained with TOPRO for cell nuclei. Autofluorescence signal is also visible. Cyan rectangle on panel (A) and cyan lines on panel (C) indicate x, y positions of panels (B,D,E).