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. 2021 Jul 29;9:692617. doi: 10.3389/fcell.2021.692617

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Copyright © 2021 Perin, Rossetti, Ricci, Cossellu, Lazzarini, Bethge, Voigt, Helmchen, Batti, Gantar and Pizzala.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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MHS-LHS junction after removal of temporal bone. (A) Overlay of plexus vascular segmentation with a substack of unsegmented hindbrain. Red, LHS; green, SS and MHS. The stippled area in yellow corresponds to the location of the missing part of the CP. Sagittal view. (B) Similar to (A), resliced to horizontal plane. Scale bar: 1 mm. (C) COLM image of a contact region between CP and DCN in a sample where temporal bone has been removed. The plexus appears ripped (arrow) but contact with DCN is not removed. Scale bar: 200 μm. (D) Magnification of the CP-DCN contact (same animal as in C) showing the indentation marks left by CP villi on DCN ependymal surface. In some positions (arrows) the CP appears connected to the DCN surface. Scale bar: 100 μm.