FIGURE 7.

Hydrogen peroxide (H₂O₂), another pathway of endothelium-dependent hyperpolarization, participates in the vasodilator response induced by G-1 in both sexes. Effect of non-selective nitric oxide synthase inhibition with N^ω-nitro-L-arginine methyl ester (L-NAME, 300 μM), cyclooxygenase inhibition with indomethacin (INDO, 10 μM) and an enzyme that specifically decomposes H₂O₂ (Catalase, 1000 units/mL) in (A) Male (n = 6) and (B) Female (n = 4) SHR groups. (C) Area under the curve (AUC) before and after L-NAME, INDO, and catalase incubation. Values are expressed as mean ± SEM. *P < 0.05 compared to the same dose in the control curve, ⁺P < 0.05 compared to the AUC of the Male SHR control curve and ^#P < 0.05 compared to the AUC of the Female SHR control curve. The curve analysis was carried out point-by-point through two-way ANOVA, followed by the Sidak post hoc test. AUCs were evaluated through one-way ANOVA followed by Tukey post hoc test.