TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristics | Reference |
|---|---|---|
| Escherichia coli | ||
| DH5α λpir | Host for π requiring plasmids | Laboratory collection |
| SM10 λpir | Host for π requiring plasmids, conjugal donor | Liang et al. (2003) |
| BL21(DE3) | Host strain for protein expression | Novagen |
| pET28a‐cadC/BL21 | BL21, pET28a carrying the cadC N‐terminal, Kmr | This study |
| Vibrio parahaemolyticus | ||
| RIMD2210633 | Clinical isolate. Carbr | Laboratory collection |
| ΔcadA | RIMD2210633, in‐frame deletion in cadA, Carbr | This study |
| ΔcadB | RIMD2210633, in‐frame deletion in cadB, Carbr | This study |
| ΔcadC | RIMD2210633, in‐frame deletion in cadC, Carbr | This study |
| cadC + | ΔcadC, pMMB207 expressing cadC‐his, Carbr, Cmr | This study |
| Plasmids | ||
| pDM4 | Suicide vector, pir dependent, R6K, SacBR, Cmr | Wang et al. (2002) |
| pMMB207 | IncQ lacIq Δbla Ptac‐lac lacZa, Cmr | Morales et al. (1991) |
| pET28a | Expressing vector, Kmr | Novagen |
| pDM4‐cadA | pDM4 with cadA fragment deleted 4 to 2,130 nt, Cmr | This study |
| pDM4‐cadB | pDM4 with cadB fragment deleted 4 to 1,338 nt, Cmr | This study |
| pDM4‐cadC | pDM4 with cadC fragment deleted 4 to 450 nt, Cmr | This study |
| pMMB207‐cadC‐his | pMMB207 carrying the cadC‐his, Cmr | This study |
| pET28a‐cadC | pET28a carrying the cadC N‐terminal, Kmr | This study |