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. 2021 May 4;16(14):2206–2210. doi: 10.1002/cmdc.202100113

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© 2021 The Authors. ChemMedChem published by Wiley-VCH GmbH

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Ternary complex formation between Brd4^B2, VCB and PROTAC GNE‐987 measured by native nESI‐FT‐ICR‐MS. Apo‐Brd4^B2 (light blue) and PROTAC‐bound Brd4^B2 (darker blue) were observed with charge states 7+ and 8+, apo−VCB (yellow) and PROTAC‐bound VCB (orange) were observed with charge states 12+ and 13+, and ternary complex (purple) was detected with charge states 14+, 15+ and 16+. PROTAC concentrations are indicated on the spectra. Quantification of each species as a ratio of total protein in the spectrum is shown on the right.