Fig 3.

SLN124 reduces liver iron overload in Hbb^th3+/− mice. (A) Representative images of Perls’ Prussian blue‐stained liver and spleen sections from Hbb^th3+/− and wild type (WT) mice treated with 3 mg/kg SLN124 alone or in combination with deferiprone (DFP). Laser ablation‐inductively coupled plasma‐mass spectrometry (LA‐ICP‐MS) iron measurements from the (B) Liver and (C) Spleen tissue sections obtained 21 days after the final injection from either treated and vehicle control groups and (D) total amount of iron per spleen. Results are presented by box plot, n = 4–7 mice/group. Statistical significance was determined in a Brown–Forsythe test (F*‐test) followed by Dunnett’s T3 post hoc tests against associated vehicle control group and as indicated by the brackets [not statistically significant (ns), P > 0·05; *P ≤ 0·05; **P ≤ 0·01; ***P ≤ 0·001]. WP, white pulp; RP, red pulp. Scale bar represents 100 µmol/l at ×20. [Colour figure can be viewed at wileyonlinelibrary.com]