Fig 7.

Tentative pathway for methoxydotrophic growth in A. fulgidus. RPKM and log₂ fold change values for all proteins shown in this figure are included in Supporting Information Table S1. Genes encoding the following enzymes can be found in A. fulgidus VC‐16: Formylmethanofuran dehydrogenase FwdA‐F (AF_0177, AF_1644, AF_1649‐51, AF_1928‐31), formylmethanofuran‐tetrahydromethanopterin formyl‐transferase Ftr (AF_2073 & AF_2207), methenyl‐tetrahydromethanopterin cyclohydrolase Mch (AF_1935), methylenetetrahydromethanopterin dehydrogenase Mtd (AF_0714), 5,10‐methylenetetrahydromethanopterin reductase Mer (AF_1066 & AF_1196), acetyl‐CoA decarbonylase/synthase CODH/ACS complex CdhABCDE (AF_0376/7/9, AF_1100/1, AF_2397/8), acetyl‐CoA synthetase Acs1‐5 (AF_0197, AF_0366, AF_0677, AF_0975, AF_0976) and acetate‐CoA ligase Acl (AF_1211 & AF_1938), pyruvate ferredoxin oxidoreductase PorABDG (AF_1699–1702), pyruvate formate lyase PflCD (AF_1449/50), PflX (AF_1961) and Act‐1‐4 (AF_0117, AF_0918, AF_1330, AF_2278), D‐lactate dehydrogenase Dld (AF_0394 & AF_0808), L‐lactate dehydrogenase LldD (AF_0807) and LldEFG (AF_0809‐AF_0811), lactate permease lctP (AF_0806), pyruvate carboxylase PycA (AF_0220) and oxaloacetate decarboxylase Oad (AF_1252), malate dehydrogenase MdhA (AF_0855) and malate oxidoreductase Mae (AF_1727), fumarate hydratase Fum‐1/2 (AF_1098/9), succinate dehydrogenase SdhA‐D (AF_0681‐4), succinyl‐CoA synthetase SucCD (AF_1539/40 & AF_2185/6), 2‐oxoglutarate/2‐oxoacid ferredoxin oxidoreductase KorABDG (AF_0468‐71), glutamate synthase GltB1‐3 (AF_0952‐4), aspartate aminotransferase AspBC (AF_0409, AF_1623, AF_2129, AF_2366, AF_1417), isocitrate dehydrogenase Icd (AF_0647), aconitase Acn (AF_1963), citrate synthase CitZ (AF_1340), sulfate adenylyltransferase Sat (AF_1667), adenylylsulfate reductase AprAB (AF_1669‐70), dissimilatory sulfite reductase DsrAB (AF_0423‐4), DsrC (AF_2228), quinone‐modifying oxidoreductase QmoABC (AF_0661‐3), molybdopterin oxidoreductase DsrMKJOP (AF_0499‐503), DsrMK(K) (AF_0543‐5), F₄₂₀H₂:quinone oxidoreductase FqoJKMLNABCDHIF (AF_1823‐33), soluble heterodisulfide reductase HdrABC (AF_1375‐7), F₄₂₀‐non‐reducing hydrogenase VhuADG (AF_1372‐4), F₄₂₀‐nonreducing hydrogenase VhtACDG (AF_1378‐81), ATP synthase AtpA‐K (AF_1158‐68), cobalamin binding protein MtoC (AF_0006) and its activator MtoD (AF_0010), O‐demethylase MtoB (AF_0007) and methyl transferase MtoA (AF_0009), MFS transporters (AF_0008 & AF_0013). H₄MPT: tetrahydromethanopterin, MQH₂: reduced menaquinone (MQ), MFR: methanofuran, Fd: ferredoxin, F₄₂₀H₂: reduced coenzyme F₄₂₀, MFS: major facilitator superfamily transporter. Expression ratio shown for A. fulgidus grown on MP versus lactate. [Color figure can be viewed at wileyonlinelibrary.com]