Table 2.

Comparison of the optimal thresholds for each data set with the suggested threshold by the developers

Tool	ABCA4 NCSS	ABCA4 DI	MYBPC3 NCSS	Suggested threshold
CADD	2.66	0.24	2.09	5ʹ extended: 7.39, 3ʹ intronic: 0.0964, exonic: 0.39
DSSP	0.01	0.13	0.01	0.30
GeneSplicer	0.18	0.05	0.21	–
MaxEntScan	0.26	0.31	0.24	0.10
MMSplice	1.42	–	1.37	2
NNSPLICE	0.13	0.40	0.30	0.05
Spidex	0.86	–	1.72	5
SpliceAI	0.19	0.18	0.11	0.20
SpliceRover	0.18	0.26	0.10	–
SpliceSiteFinder‐like	0.01	0.12	0.09	0.05