FIGURE 2.

Effect of TIX‐5 variants on TF‐initiated thrombin generation. Thrombin generation was initiated with 5 pM TF in pooled normal human plasma (NHP) in the absence or presence of 4 μM TIX‐5. (A‐C) Thrombograms representing NHP in the absence of TIX‐5 (control, blue), WT TIX‐5 (red), TIX‐5 R35A/A38 K (beige), TIX‐5 E99Q (orange), TIX‐5 E147Q/E151Q (green), TIX‐5 E99Q/E147Q/E151Q (dark red), TIX‐5 I64A/L65A (purple), TIX‐5 I126A/V127A (gray), and TIX‐5 K184A (dark blue). (D‐E) Lagtimes of the thrombograms in the absence or presence of TIX‐5 are shown. The data represent means ± SD of eight replicates. *p < .05, **p < .01, *** p < .005. SD, standard deviation; TF, tissue factor; TIX, tick inhibitor of factor Xa