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. 2021 May 1;19(7):1697–1708. doi: 10.1111/jth.15329

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© 2021 The Authors. Journal of Thrombosis and Haemostasis published by Wiley Periodicals LLC on behalf of International Society on Thrombosis and Haemostasis

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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Effect of TIX‐5 variants on FV activation by FXa. (A) FV‐1033 activation was initiated by 1 nM FXa in the presence of phospholipids and evaluated by western blotting with an anti‐FV heavy chain antibody. The FV‐1033 activation products following cleavage at Arg709 (the FV heavy chain, FV hc), Arg1018 (FV‐1033(a)1018), and Arg1545 (FV‐1033(a)1545) and the reaction time points in minutes are indicated. (B) Band intensities of the FV‐1033(a) activation products at 4 and 8 min and of the FV heavy chain (HC) at 8 min were quantified and plotted. The mean intensity in the absence of TIX‐5 was set at 100% and the mean intensity in the presence of WT TIX‐5 at 0%. FV, factor V; FXa, factor Xa; TIX, tick inhibitor of factor Xa