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. 2021 May 7;16(13):2089–2093. doi: 10.1002/cmdc.202100067

Table 4.

The biological data of the SFC‐separated compounds and analytical samples over four days.

IC50m] [a]

Days of incubation

PfIspE[b]

EcIspE

PK/LDH

PfNF54

0

>500

>500

n.d.

n.d.

1

>500

>500

n.d.

n.d.

2

>500

>500

n.d.

3.5±0.3

3

486±20

397[c]

n.d.

n.d.

4

99±23

75±16

95±21

2.7±0.3

DP1 “380”

199±26

n.d.

>500

n.i. (>20)

DP2 “365”

59±4

n.d.

37±4

2.1±0.2

[a] All compounds were dissolved in methanol shortly before the assay. [b] Freshly dissolved compound 1 (PfIspE IC50=>500 μm). [c] Error given as formal standard error. n.d.: not determined, n.i.: no inhibition at the highest concentration tested, PK/LDH: pyruvate kinase and lactate dehydrogenase, Pf: Plasmodium falciparum, Ec: Escherichia coli, DP: decomposition product.