FIGURE 3.

Schematic model of POMGNT2 and mannosyl peptide. Conservation of amino acid sequences of the mannosylated sites of α‐DG at around residue 379 is presented at the bottom of the figure. The sequence alignment of 23‐mer sequences of α‐DG from the same vertebrate orthologues of previous report (Halmo, 2017) was carried out by ConSurf program (Berezin, 2004). The amino acid sequence of the 379Man long peptide, which was used in this study, is on it. The number corresponding to the 379Man short peptide is shown between them. Areas shown in blue and brown boxes are regions interacting by the catalytic domain and FnIII domain of POMGNT2, respectively. Note that the two domains are from different molecules in the dimer