Figure 1.

Analysis of fatty acids (FA) and glycerolipids in transgenic Phaeodactylum tricornutum overexpressing acyl‐ACP Δ9‐desaturase.

(a) Quantitative analysis of FA composition in total lipids (µg FA 10⁶ cells⁻¹) in wild‐type (WT) and transgenic palmitoyl‐ACP Δ9‐desaturase (PAD) lines during the E phase. DHA, docosahexaenoic acid; EPA, eicosapentaenoic acid.

(b) Glycerolipid classes comparison from WT and transgenic PAD lines during the E phase of growth (3 days). Insert: quantitative analysis of total glycerolipids (nmol of lipid 10⁶ cells⁻¹). Values expressed are relative percentage of total lipids. Growth stage was determined by analyzing cell density. Values presented are the average of three biological replicates, error bars represent SE. Asterisks indicate significant difference (P < 0.05, LSD) relative to WT. DAG, diacylglycerols; DGDG, digalactosyldiacylglycerol; DGTA, diacylglycerylhydroxymethyltrimethyl‐β‐alanine; MGDG, monogalactosyldiacylglycerol; PC, phosphatidylcholine; PE, phosphatidylethanolamine; PG, phosphatidylglycerol; PI, phosphatidylinositol; SQDG, sulfoquinovosyldiacylglycerol; TAG, triacylglycerols.