Figure 1.

Schematic of the two‐layered vesicle generation setup. Lipids are dissolved in the oil phases and self‐assemble at all water/oil interfaces. A stable multilayered water/oil/water/oil column is generated by ensuring each layer is denser than the one above it. Water‐in‐oil emulsion droplets, which are the densest solution in the system, are added to the top of the column. These are driven through the interface under centrifugation, picking up sequential monolayers to generate bilamellar vesicles at the bottom. Three‐layered vesicles can be formed in a two‐step process by performing an additional centrifugation round.