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. 2021 Jul 29;9:735149. doi: 10.3389/fchem.2021.735149

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Copyright © 2021 Liao and He.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A–B) Analytical HPLC and mass analysis of SelF(1–41)-MPAA (1) and SelF(42–74)-MPAA (2). 1: observed mass 4,593.0 Da, calcd 4,593.0 Da (average isotopes); 2: obsd. mass 4,067.0 Da, calcd. mass 4,066.9 Da (average isotopes). (C) Overexpression and Ulp1 cleavage to afford SelF(75–134) (3). (D) Analytical HPLC and mass analysis of SelF(75–134) (3) with the obsd. mass 6,965.7 Da, calcd. mass 6,965.7 Da (average isotopes). Detailed reaction conditions in the Materials and Methods section.