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. 2021 Jul 29;9:735149. doi: 10.3389/fchem.2021.735149

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Copyright © 2021 Liao and He.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) Analytical HPLC traces of ligation and refolding reaction of SelF(U65C/Q74A). (B) Mass analysis of ligation reaction product 6 and its folded protein 7, respectively. 6: obsd. mass 14,976.0 Da, calcd. mass 14,976.2 Da (average isotopes); 7: obsd. mass 14,967.6 Da, calcd. mass 14,968.2 Da (average isotopes). (C) Analytical SDS-PAGE analysis of the ligation reaction. (D,E) CD spectra of the fully synthetic SelF(U65C/Q74A) (7) (D) and the recombinant SelF (U65C) (8) (E).