Skip to main content
. 2021 Jul 29;9:735149. doi: 10.3389/fchem.2021.735149

TABLE 1.

Disulfide bond elucidation through the enzymatic digestion of SelF(U65C) (8), SelF(U65C/C42S)-(CAM) (9) and SelF(U65C/C43S)-(CAM) (10).

Entry Protein variant Digestion Sequences Molecular weight (Da)
Obs Calc
1 SelF(U65C) (8) Trypsin graphic file with name fchem-09-735149-fx2.jpg 1,336.6 1,336.7
2 Trypsin and chymotrypsin graphic file with name fchem-09-735149-fx3.jpg 1,060.7 1,060.5
3 graphic file with name fchem-09-735149-fx4.jpg 1,583.0 1,583.7
4 SelF(U65C/C42S)-CAM (9) Trypsin graphic file with name fchem-09-735149-fx5.jpg 2,557.0 2,556.7
5 Trypsin and chymotrypsin graphic file with name fchem-09-735149-fx6.jpg 1,243.0 1,242.5
6 SelF(U65C/C43S)-CAM (10) Trypsin graphic file with name fchem-09-735149-fx7.jpg 1,259.0 1,259.2
7 Trypsin and chymotrypsin graphic file with name fchem-09-735149-fx8.jpg 2,180.9 2,181.3