Table 3.
Energy penalty for mitochondria with lamellar and tubular cristae.
| A) Crista Shape | KINASE (rel) |
J(AS) (mol/μm2/ms) |
SCRIS (μm2) |
ATP OUTPUT q = J(AS) × SCRIS (mol/ms) |
ENERGY PENALTY Δq = [Jmax(AS) - J(AS)] × SCRIS (mol/ms) |
Δq/qmax (%) |
q/qo |
|---|---|---|---|---|---|---|---|
| LAM | SLOW | 74.5 | 4.8 | 358 | 118 | 25 | 2.0 |
| FAST | 85.5 | 410 | 65 | 14 | 2.3 | ||
| TUB | SLOW | 84 | 4.1 | 344 | 61.5 | 15 | 1.9 |
| FAST | 90 | 369 | 37 | 9 | 2.1 | ||
| NONE (o) | SLOW | 99 | 1.8 | 178 | 0 | 0 | 1 |
| FAST | 99 | 178 | 0 | 0 | 1 |
| B) Crista Shape | KINASE (rel) |
J(AS) (mol/μm2/ms) |
SCRIS (μm2) |
ATP OUTPUT q = J(AS) × SCRIS (mol/ms) |
ENERGY PENALTY Δq = [Jmax(AS) - J(AS)] × SCRIS (mol/ms) |
Δq/qmax (%) |
q/qo |
|---|---|---|---|---|---|---|---|
| LAM | SLOW | 38 | 37.4 | 1421 | 2281 | 62 | 4.2 |
| FAST | 67 | 2506 | 1197 | 32 | 7.4 | ||
| TUB | SLOW | 59 | 20.4 | 1204 | 836 | 41 | 3.6 |
| FAST | 74 | 1510 | 510 | 25 | 4.5 | ||
| NONE (o) | SLOW | 99 | 3.4 | 337 | 0 | 0 | 1 |
| FAST | 99 | 337 | 0 | 0 | 1 |
Effects of crista morphology and kinase activity on ATP output (q) for lamellar (LAM) and tubular (TUB) spatial models of the mitochondrial inner membrane, relative to a model with no cristae (NONE). Results in Table A (upper) correspond to fluxes of ATP synthase, J(AS), and IM surface areas, SIM, for the largest 3D spatial models used for computer simulations with the reduced BioModel. Results in Table B (lower) correspond to J(AS) values extrapolated for rat cardiac muscle mitochondria (see text), using the linear regression equations in Fig. 6B. Kinase activities are labelled SLOW and FAST, corresponding to forward reaction constants (kf) of 0.003 and 0.009 msec−1, respectively. Other abbreviations: mol = molecules, ms = millisecond, qo = ATP output for the model with no cristae, qmax = hypothetical q for a model with cristae calculated using J(AS) for the model with no cristae, Jmax(AS).