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. Author manuscript; available in PMC: 2021 Aug 12.

Published in final edited form as: Int J Phys Med Rehabil. 2020 Nov 30;9(1):582.

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This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Figure 1: — Strategies for cell type specific sgRNA expression. Cell type specific sgRNA expression can be achieved by 1) the combination of a cell type specific RNA polymerase-II (Pol-II) promoter and sgRNA flanked with self-cleaving ribozymes: e.g. hammerhead (HH) ribozyme and hepatitis delta virus (HDV) ribozyme, 2) transcript processing by cell type specific miRNA or 3) the combination of cell type specific Pol-II promoter and sgRNA flanked with a mutant transfer RNA (Mut tRNA) which has minimal promoter activity and can maximize processing activity.

Abbreviation: pA: Polyadenylation Signal.