Fig 5. TATTW-containing octanucleotide sequence in relation to strength of viral promoter transcription.
(A) Scatterplot of viral TSR strength (number of nascent RNA reads) versus effect of UL87HF LTF depletion on TSR strength (dTag vs. CTRL treatments) for all active viral TSRs (open circles). UL87 LTF-responsive TSRs divided into top, mid, and bottom tertiles based on individual-level viral TSR strength (red hatched lines). Viral TSRs having a TATT positioned -40 to -28 upstream of the MAXTSS are marked in red. Data derived from the experiment and method of measurement described in Fig 3B. (B) Analysis of core promoter elements by tertile rank in TSR strength. MEME algorithm was applied to positions -50 to -20 relative to MAXTSS for UL87 LTF-responsive TSRs having an upstream TATT. WebLogo was applied to positions -4 to +4 relative to MAXTSS.