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. 2021 Aug 2;17(8):e1009796. doi: 10.1371/journal.ppat.1009796

Fig 7. Noncanonical sequences in LTF-responsive promoters.

Fig 7

(A) Proportional distribution of different motif types in LTF-responsive promoters by tertile rank in TSR strength. Based on effects of UL79HF LTF depletion (6-h dTag treatment) on viral TSR strength at 72 hpi. Null, unclassified sequence. (B) MEME logo determination for UL87 LTF-responsive TSRs having a TATAT positioned -50 to -20 relative to MAXTSS by tertile rank in TSR strength. (C-E) Effect of UL79HF LTF depletion on viral TSR strength at 72 hpi versus baseline TSR strength (C and E) or PSI (D) for viral TSRs ≥435 nascent RNA reads (open circles). Viral TSRs with TATT (red), TATAT (pink), TATAA (blue), TGTT (green), or YRYT (violet) sequences positioned -40 to -28 relative to MAXTSS are marked with dots colored accordingly. Horizontal dashed line divides top and mid TSR strength tertiles. (F) Effect of dTag treatment for 2 versus 6 h on viral TSR strength (dTag/CTRL) at 96 hpi for HCMV UL87HF. Viral TSR strength was determined by spike-in normalized PRO-Seq-Flavo method. Arrows point to viral asUL49 and RL10 TSRs having upstream TGTT and TACT sequences, respectively.