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. 2021 Aug 12;16(8):e0255295. doi: 10.1371/journal.pone.0255295

Fig 1. Overview of experimental design.

Fig 1

A. Male and female mice were first tested individually for their response to bark playbacks. Based on their response they were paired using a 2 x 2 factorial design using level of approach and sex as factors. After 10–11 days post pairing, the pairs were tested again for their response as a dyad. Spectrograms showing the acoustic characteristics of B) Barks, C) Sweeps and D) Sustained vocalizations.