Fig 2. Rotavirus susceptibility of wild-type and IFN receptor-deficient C57BL/6 mice is greatly enhanced if the microbiome is depleted with antibiotics.
WT (n = 7), Ifnar1-/- (n = 6), Ifnlr1-/- (n = 8) and Ifnar1-/- Ifnlr1-/- (DKO, n = 10) mice were left untreated or treated with antibiotics (Abx) before oral infection with 104 SD50 of the EC strain of murine rotavirus. On day 5 post-infection, fecal pellets were collected and viral RNA levels were determined by RT-qPCR. Symbols represent individual mice, and bars represent means ± SEM. Line represents limit of detection of the RT-qPCR assay. Statistical analysis: Ordinary one-way ANOVA with Tukey’s multiple comparisons; ****p<0.0001.