Figure 6.

Modulation of afferent Ca²⁺ influx through VGCC by thapsigargin and ryanodine. A, Mean traces of the Ca²⁺ transients obtained with 300-ms depolarization to +20 mV before (top) and during perfusion of thapsigargin (bottom). On the right, average peak Ca²⁺ level (ΔF) for step depolarizations. B, Mean traces of the Ca²⁺ transients during with steps to +20 mV, using intracellular solutions containing DMSO or ryanodine 1 μm. Average peak Ca²⁺ signal (ΔF/F₀) are shown on the right for experimental conditions in low and high concentrations of ryanodine (Ry; 1 and 100 μm) and dantrolene (30 μm). Wilcoxon signed-rank test; *p < 0.05.