Figure 4.

The effects of overexpressed or silenced TAZ on the expressions of osteoblast differentiation, osteogenesis-related genes, and TAZ. (a) Alizarin staining was used to identify the differentiation of stem cells into osteoblasts, under 200 × magnification. Mineral accumulation was quantified. (b–d) The mRNA expressions of osteoblast-related genes were analyzed by qRT-PCR. β-Actin was employed as an internal control. (e and f) TAZ protein expression in cytoplasm and nucleus was detected by Western blot. β-Actin and Lamin B1 were employed as internal controls. All experiments were performed in independent triplicate. ^∗∗p < 0.01, ^∗∗∗p < 0.001 vs. vector; ^#p < 0.05, ^##p < 0.01, ^###p < 0.001 vs. CGF + vector; ^{^}p < 0.05, ^{^^}p < 0.01 vs. shTAZ; ^&p < 0.05, ^&&p < 0.01 vs. pc-TAZ.