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. 2021 Aug 5;2021:7407086. doi: 10.1155/2021/7407086

Figure 3.

Figure 3

miR-486 targets CADM1. (a) Starbase V2.0 to predict the binding site between miR-486 and CAMD1; (b) dual-luciferase reporter assay to detect the targeting relationship between miR-486 and CADM1; (c) qRT-PCR assay to detect CADM1 expression in normal and OC tissues. CADM1 expression is low in OC tissues; (d) Western blot to detect the expression of CADM1 protein in normal and OC tissues; (e) qRT-PCR to detect the expression of CADM1 in SKOV3, Caov3, ES-2, OVCAR3, and IOSE80; (f) qRT-PCR to detect the expression of CADM1 mRNA in SKOV3 cells after transfection with miR-486 inhibitor; (g) Western blot to detect the expression of CADM1 protein in SKOV3 cells after transfection with miR-486 inhibitor; (h) Pearson analysis of the correlation between miR-486 and CADM1. ∗P < 0.05 and ∗∗P < 0.01.