Fig. 3.

Treatment with IL-36γ-armed OV increased the number of T cells in MC38 solid colon tumor tissue. B6 mice were subcutaneously inoculated with 5.0e5 MC38 cancer cells. When the tumor size reached ~ 5 mm in diameter, PBS, vvDD, vvDD-IL-36γ (1.0e8 pfu per tumor) was injected intratumorally (n = 6 ~ 8/group). a Ten days post-treatment, tumor tissues were collected, fixed, and stained for CD3, CD4, CD8, and DAPI. Representative images from each group were presented. b Statistics of the percentages of CD3⁺ T cells, CD3⁺CD4⁺ T cells, and CD3⁺CD8⁺ T cells per area, ** p < 0.01, *** p < 0.001, **** p < 0.0001