REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
GFP Monoclonal (GF28R)	Thermo Fisher Scientific/Invitrogen	Cat# MA5-15256
GFP Polyclonal	Thermo Fisher Scientific/Invitrogen	Cat# A11122; RRID: AB_221569
Phospho-myosin light chain 2 (S19)	Cell Signaling	Cat# 3671; RRID: AB_330248
Cleaved Caspase-3 (Asp175)	Cell Signaling	Cat# 9661; RRID: AB_2341188
C-cadherin	Developmental Studies Hybridoma Bank	6B6
Phospho-ERK1/2	Sigma Aldrich	Cat# E7028; RRID: AB_259347
ERK1/2	Cell Signaling	Cat# 9102S; RRID: AB_330744
α-tubulin	Sigma Aldrich	Cat# T9026; RRID: AB_477593
Alexa Fluor 488 goat anti-mouse	Thermo Fisher Scientific/Invitrogen	Cat# A11001; RRID: AB_2534069
Alexa Fluor 568 goat anti-mouse	Thermo Fisher Scientific/Invitrogen	Cat# A11004; RRID: AB_2534072
Alexa Fluor 488 goat anti-rabbit	Thermo Fisher Scientific/Invitrogen	Cat# A11008; RRID: AB_143165
Alexa Fluor 568 goat anti-rabbit	Thermo Fisher Scientific/Invitrogen	Cat# A11011; RRID: AB_143157
Alexa Fluor 568 Tyramide Reagent	Thermo Fisher Scientific/Invitrogen	Cat# B40956
Goat anti-Rabbit IRDye800CW	Abcam	Cat# 216773
donkey anti-mouse IRDye680RD	Abcam	Cat# 216778
Bacterial and virus strains
Subcloning Efficiency DH5α Competent Cells	Thermo Fisher Scientific	18265017
Chemicals, peptides, and recombinant proteins
PMSG-Intervet (Pregnant Mare Serum Gonadotrophin)	Intervet UK	N/A
Chorulon (Human Chorionic Gonadotrophin)	Intervet UK	N/A
MS222 – Ethyl 3-aminobenzoate methanesulfonate salt	Merck	A5040-100G
Phenol:Chloroform:IAA, 25:24:1	Thermo Fisher Scientific	AM9730
L-cysteine	Sigma Aldrich	168149
Ficoll	Sigma Aldrich	PM400
Protease inhibitor cocktail	Promega	G6521
PhosSTOP phosphatase inhibitor	Sigma Aldrich	4906845001
DAPI	Thermo Fisher Scientific	D1306
NotI	New England Biolabs	R0189L
Critical commercial assays
mMessage mMachine SP6 transcription kit	Thermo Fisher Scientific	AM1340
PureLink Quick Plasmid Miniprep Kit	Thermo Fisher Scientific	K210010
Experimental models: Organisms/strains
Mature female Xenopus laevis	Bred in-house and from European Xenopus Resource Centre (EXRC).	https://xenopusresource.org/
Mature male Xenopus laevis	Bred in-house and from European Xenopus Resource Centre (EXRC).	https://xenopusresource.org/
Oligonucleotides
Morpholino: MHC-B (Myosin Heavy Chain-B, myosin II) 5′-CTTCCTGCCCTGGTCTCTGTGACAT-3′	Gene Tools LLC60	N/A
Morpholino: Standard control 5′-CCTCTTACCTC AGTTACAATTTATA-3′	Gene Tools LLC	Product name “Standard Control oligo”
kRasV12FwdBspEI: 5′-CTGATCCGGAATGACTGAATATAAACTTGT-3′	Eurogentec	N/A
kRasV12RvsXhoI: 5′-GCTACTCGAGTTACATAATTACACACTTTG-3′	Eurogentec	N/A
MycFwdBspEI: 5′-AATATCCGGAATGCCCCTCA-3′	Eurogentec	N/A
MycRvsXhoI: 5′-ATACCTCGAGTTACGCACAAGAGTTC-3′	Eurogentec	N/A
Recombinant DNA
mCherry-Histone2B in pCS2+	Kanda et al.66	N/A
BFP-CAAX in pCS2+	Bement Lab, University of Wisconsin-Madison.67	N/A
N-GFP in pCS2+	Bement Lab, University of Wisconsin-Madison68	N/A
GFP-kRasV12 in pCS2+	Human kRasV12 from Addgene cloned into N-GFP/pCS2+	Addgene 2544
GFP-cMYC in pCS2+	Human cMYC-IRES-GFP from Addgene cloned into N-GFP/PCS2+.	Addgene 8119
GFP-RhoAQ63L in pcDNA3	Addgene	Addgene 12968
mCherry-UtrCH in pCS2+	Bement Lab, University of Wisconsin-Madison68	N/A
Software and algorithms
Fiji/ImageJ Version 2.0.0-rc-69/1.53a	NIH	https://imagej.nih.gov/ij/
GraphPad Prism 9	GraphPad Software	https://www.graphpad.com/scientific-software/prism/
Python v3.6.5 – in-house python scripts implementing watershed algorithm.	Python Core Team	https://www.python.org/
Vertex-based model	Nestor-Bergmann et al.3,69	N/A