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. 2021 Aug 12;12:4878. doi: 10.1038/s41467-021-25195-1

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Growth curves of eIF6^+/+ and eIF6^+/− mice fed with high-fat diet, HFD (n = 12 for eIF6^+/+ and n = 15 for eIF6^+/−). Data are expressed as means ± SD. Two-way ANOVA. ***p value ≤ 0.001. b Body weight gain is reduced in eIF6^+/− mice compared with wt ones at the indicated time points (n = 12 for eIF6^+/+ and n = 14 for eIF6^+/−). Two-tailed t test. Data are represented as means ± SD. c Blood triglycerides and cholesterol levels are reduced in eIF6^+/− mice at the end of HFD regimen (n = 11 for genotype). Two-tailed t test. Data are represented as means ± SD. d Glucose levels in the blood: insulin tolerance test shows that eIF6^+/− mice respond better to insulin administration at 120 min (n = 5 for genotype). Two-tailed t test. Data are represented as means ± SD. e Outline of the experiment. Representative images of eIF6^+/+ and eIF6^+/− livers are shown. Right: representative western blotting analysis of eIF6 levels in HFD-fed eIF6^+/+ and eIF6^+/− mice. f Liver to body weight ratio shows that eIF6^+/− livers are less enlarged compared with wild-type ones at the end of HFD feeding. Two-tailed t test. Data are represented as means ± SD. (n = 18 for eIF6^+/+ and n = 19 for eIF6^+/−). g Representative images of liver sections: staining with Oil Red O reveals reduced lipid droplets accumulation in eIF6^+/− mice compared to wild-type ones. Scale bar = 20 μm. h Collagen fibers stained with Sirius Red: liver fibrosis is reduced. Scale bar = 20 μm. i Quantification of the area stained by Oil Red O. Data are expressed as a percentage of eIF6^+/+ controls. Hepatic lipid content is decreased in eIF6^+/− mice. (n = 4 for genotype). Two-tailed t test. j Representative frequency distribution of lipid droplets (LDs) size. Larger LDs were detected in eIF6^+/+ livers compared with eIF6^+/− ones. k Quantification of area stained by Sirius Red. Hepatic fibrosis is decreased in eIF6^+/− mice. (n = 3 for genotype). Data are provided as a Source Data File.