Table 1.
Temporal expression of SfAV and TnAV genes coding for abundant virion structural proteins and enzymes in tissues of their respective hosts, larvae of Spodoptera frugiperda and Trichoplusia ni, at 7, 14, and 21 days post-infection.
| SfAV genes for structural proteins and enzymes | Somatic | Hemolymph | ||||
|---|---|---|---|---|---|---|
| 7 | 14 | 21 | 7 | 14 | 21 | |
| ORF009 (DEAD-like helicase of the SNF2 family) | 223.28 | 132.15 | 115.88 | 429.58 | 546.50 | 167.04 |
| ORF041 (Major capsid protein) | 560.29 | 593.22 | 785.83 | 986.79 | 1708.50 | 826.11 |
| ORF048 (DNA condensation, P64) | 94.95 | 74.56 | 86.84 | 155.62 | 278.25 | 93.05 |
| ORF061 (Ervl/Alr family protein) | 9.97 | 19.94 | 29.28 | 12.41 | 36.49 | 23.66 |
| ORF064 (Serine/threonine protein kinase) | 53.70 | 78.87 | 113.49 | 42.29 | 139.32 | 109.23 |
| ORF075 (S1/P1 nuclease) | 472.73 | 260.86 | 194.99 | 733.32 | 815.64 | 349.90 |
| ORF084 (Dynein-like β chain) | 12.73 | 20.23 | 17.58 | 13.13 | 43.85 | 26.89 |
| ORF091 (High-mobility-group protein/yabby-like protein) | 2060.79 | 2708.71 | 5202.51 | 6964.89 | 14,836.89 | 5624.16 |
| ORF109 (Haloacid dehalogenase-like hydrolase/CTD-like phosphatase) | 140.94 | 97.69 | 105.72 | 223.98 | 332.07 | 150.18 |
| TnAV genes for structural proteins and enzymesa | ||||||
| ORF161 (DEAD-like helicase/SWI/SNF2 family helicase) | 3.28 | 3.62 | 1.27 | 175.55 | 64.06 | 26.46 |
| ORF153 (Major capsid protein) | 185.44 | 177.17 | 70.55 | 10,696.28 | 4668.94 | 1525.08 |
| ORF118 (Erv1/Alr family protein) | 5.77 | 5.43 | 3.11 | 284.63 | 139.22 | 54.05 |
| ORF114_115 (serine/threonine protein kinase)b | 5.27 | 4.99 | 1.84 | 360.55 | 144.34 | 54.78 |
| ORF135 (S1/P1 nuclease) | 14.83 | 13.91 | 3.38 | 610.31 | 211.78 | 86.93 |
| ORF043 (Dynein-like beta chain) | 7.99 | 8.35 | 3.32 | 355.03 | 148.46 | 65.31 |
| ORF059 (Yabby-like transcription factor/HMG box) | 50.75 | 36.21 | 18.22 | 2185.46 | 1156.16 | 413.54 |
| ORF093 (Haloacid dehalogenase-like hydrolase/CTD phosphatase-like protein) | 1.33 | 2.88 | 2.21 | 80.76 | 30.29 | 18.46 |
| ORF147 (chromosome segregation protein SMC) | 20.57 | 23.00 | 8.01 | 1474.73 | 455.94 | 133.60 |
Data presented are the average of three replicates of the Reads Per Kilobase Per Million (RPKM) at each time point for somatic tissues versus hemolymph.
The primary tissue initially infected by SfAV in S. frugiperda is the fat body, whereas in T. ni the initial tissues infected by TnAV are the fat body, epidermis, and tracheal matrix.
aThe TnAV-6a (TnAV-2c, previously)23 was used as a reference genome to refer to the ORFs order in our TnAV-6a1 variant.
bThe _ sign refers to two ORFs that were found in our TnAV-6a1 variant strain as a single ORF.