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. 2021 Aug 12;11:16419. doi: 10.1038/s41598-021-95858-y

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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The percentage of blood monocytes (A) and peritoneal macrophages (B) that stained positive for the presence of ABCA1 protein, and (C) relative surface protein expression levels of ABCA1 on peritoneal macrophages in LDL receptor knockout mice transplanted with bone marrow from USF1 knockout (USF1 KO) mice or their wild-type (WT) littermate controls. (D) The extent of cholesterol efflux from WT and USF1 KO bone marrow-derived macrophages to the exogenous acceptor apolipoprotein A1. Data in panels (A–C) represent the means ± SEM of 6 randomly chosen WT and USF1 KO bone marrow recipients, whilst the in vitro data in panel (D) represent the means ± SEM of 4 cell culture wells. **P < 0.01 versus WT.