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. 2021 Aug 12;12:4902. doi: 10.1038/s41467-021-25217-y

Fig. 4. Application of OPTI-CGBEs for C-to-G editing in mice embryos.

Fig. 4

a Schematics of zygote and two-cell injection of OPTI-CGBEs. b The C-to-G transversion efficiency of OPTI-CGBEs on three target sites by zygote or two-cell injections. n = 30/30/27/27 for Tyr-A, n = 28/23/30/21 for Tyr-B, and n = 21/15/9/15 for Tyr-C, respectively (Ordered: eOPTI-CGBE-Zygote, eOPTI-CGBE-Two-cell, cOPTI-CGBE-Zygote, and cOPTI-CGBE-Two-cell). The center line indicates the median, and the bottom and top lines of the box represent the first quartile and third quartile of the values, respectively. Tails extend to the minimum and maximum values. P values were calculated by two-sided Wilcoxon rank sum tests. c Sanger sequencing results of eOPTI-CGBE on three target sites by two-cell injection. Red arrows indicated the targeted Cs. d The hair colors of F0 and F1 mice by zygote or two-cell injection of eOPTI-CGBE mRNA and sgRNA-Tyr-C. The numbers on each bar represent the number of mice. P value was calculated by Chi-square test.