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. 2021 Aug 12;12:4898. doi: 10.1038/s41467-021-24995-9

Fig. 5. ptc-3(RNAi) decreases phospholipid FA saturation and elongation.

Fig. 5

A Increasing the growth temperature from 20 °C to 25 °C improves the development of ptc-3(RNAi) animals. Representative bright-field pictures of worms on growth plates. B Quantification of ptc-3(RNAi) survivors at both temperatures. Mean and SEM error bars, unpaired one-tailed T test, = 197 (20 °C) and 246 (25 °C) animals from three independent experiments *p = 0.02. C Lipidomics on mock or RNAi-treated worms. ptc-3(RNAi) worms showed minor differences in lipid head group distribution. Mean and SEM error bars, n = 6. D Lipidomics revealed differences in lipid acyl chain composition upon ptc-3(RNAi). There is a shift from PUFAs to saturated FA and MUFAs. Mean and SEM error bars, each point represents the average of six experiments per lipid type. Statistical source data are available as source data.