Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Aug 12;11:16427. doi: 10.1038/s41598-021-95941-4

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

p300 and HDAC1 regulate Api5 localisation. MCF7 cells were treated with (a,b) 7.5 μM C646, (c,d) 0.1 nM romidepsin and (e,f) 7.5 μM C646 and 0.1 nM romidepsin for 4 h and 16 h, respectively. Immunofluorescence assay was performed using Api5 specific antibody and cells with different Api5 staining pattern were manually counted and analysed. The bar graph represents the percentage of cells with Api5 staining only in the nucleus (red), only in the cytoplasm (yellow) or in both (blue). (g) CTCF value of Api5 intensity in the nucleus and the cytoplasm was measured in the same experimental set up and the values are represented as box plots. Here, red corresponds to nuclear intensity and yellow corresponds to the cytoplasmic intensity of Api5. (h) Cells exhibiting Api5 nuclear foci following different inhibitor treatments were manually counted and represented as bar graphs showing percentage of cells with nuclear foci.