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. 2021 Aug 12;11:16427. doi: 10.1038/s41598-021-95941-4

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Acetylation status of Api5 affects cell cycle. MCF7 cells transfected with siRNA-resistant Api5-K251 mutants 24 h post Api5 knock down. Cells were fixed in ethanol and stained with propidium iodide and cell cycle profile was monitored. (a) Percentage of cells in the different cell cycle phases were analysed in a flow cytometer and plotted as box plots. (b) Western blotting was performed to check the levels of (b) Api5, PCNA, Cyclin A and Cyclin B1 and (c–f) quantified after normalising to the loading control GAPDH. (g) MCF7 cells transfected with mVenus-tagged Api5 WT and K251 mutants were counted using Sceptar™ to check for cell proliferation.