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. 2021 Aug 12;11:16409. doi: 10.1038/s41598-021-95720-1

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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NOTUM overexpression decreases adiposity in chow diet-fed mice. Male C57BL/6J mice were infected with AAV-GFP or AAV-Notum (n = 6 per group) and maintained on a low fat chow diet for 4 weeks before tissue collection. Body weight (a), fat mass (b), lean mass (c), and fat pad weights (d) are shown. Gene expression analysis by qPCR of iWAT (e), n = 6, and iBAT (f), n = 4–5, are shown. Symbols are the same as shown in Fig. 1.