Skip to main content
. 2021 Aug 12;11:16378. doi: 10.1038/s41598-021-95839-1

Figure 2.

Figure 2

IL-34 inhibited RA-FLS apoptosis by regulating IL-17. (A) Apoptosis was analyzed after the treatment of RA-FLSs with different concentrations of IL-34 (0 ng/ml, 25 ng/ml, 50 ng/ml and 100 ng/ml). The degree of apoptosis was assessed by flow cytometry using Annexin V and propidium iodide (PI) staining. (B) Quantification of apoptopic RA-FLSs. (C,D) Apoptosis was analyzed after the treatment of RA-FLSs with IL-34 (100 ng/ml) or IL-34 (100 ng/ml) + PB (4 UM). The differences between the four groups were tested by ANOVA. Statistical comparisons between two groups were performed by t-tests. The data are expressed as the mean ± SEM. **P < 0.01, ***P < 0.001 vs the 0 group; #P < 0.05, ###P < 0.001 vs the 25 group; ▲P < 0.05 vs the 50 group; $$$P < 0.001 vs the IL-34 group.