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. 2021 Aug 12;11:16430. doi: 10.1038/s41598-021-95799-6

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Detection of SARS-CoV-2 in NP samples using RT-LAMP. (A) Comparison of RT-LAMP and RT-PCR results. The Ct values (RT-PCR results) of 126 COVID-19 positive patients (y-axis) were compared to the RT-LAMP readout (x-axis) taken after 30 min of incubation at 65 °C (positive, +/yellow; negative, −/pink). The dotted red line indicates the Ct below, which there is 100% agreement between RT-LAMP and RT-PCR. (B) Sensitivity of the RT-LAMP assay across different ranges of Ct values (which reflect different viral loads). The thicker horizontal lines indicate the specificity calculated for the indicated Ct range (according to the data of panel (A) and Table 1). The vertical lines indicate the corresponding 95% confidence intervals.