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. 2021 Aug 12;11:16430. doi: 10.1038/s41598-021-95799-6

Figure 3.

Figure 3

Limit of detection of the saliva RT-LAMP assay. Spike-in experiments of a healthy donor saliva with (A) tenfold dilutions of in vitro transcribed (IVT) viral RNA (N-gene) and (B) in vitro propagated SARS-CoV-2 virions. Saliva samples were processed as described in “Materials and methods” and 2 μL were analyzed by colorimetric RT-LAMP. PFUs plaque forming units. Figures are representative of three independent experiments.