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. 2021 Aug 12;11:16430. doi: 10.1038/s41598-021-95799-6

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Detection of SARS-CoV-2 in saliva samples using RT-LAMP. RT-LAMP analysis of saliva samples of confirmed COVID-19 patients who (A) induced or not (B) salivation before sample collection. (C) Comparison of RT-LAMP and RT-PCR results. The Ct values (RT-PCR results) of 39 COVID-19 positive patients (y-axis) were compared to the RT-LAMP readout of the matched saliva samples (x-axis), after 30 min of incubation at 65 °C (positive, +/yellow; negative, −/pink). Black circles—direct saliva; orange circles—RNA extracted from saliva. (D) RNAs from the saliva of false negative samples (as determined by the direct saliva test) were extracted using plasmid DNA miniprep columns (ZR Plasmid Miniprep-Classic Kit, Zymo Research) and re-analyzed. NTC no template control. Cts (N gene) obtained for the paired NP samples are indicated.