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. 2021 Aug 12;11:16430. doi: 10.1038/s41598-021-95799-6

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Analytical sensitivity of an in-house-made colorimetric RT-LAMP assay. Tenfold dilutions of in vitro transcribed (IVT) viral RNA (N-gene) were amplified via RT-LAMP and detected using a colorimetric buffer together with (A) RTx (New England Biolabs) and Bst LF (homemade), (B) MashUP RT (homemade) and Bst 2.0 (New England Biolabs) or (C) MashUP RT (homemade) and Bst LF (homemade). The in-house-made setup was next used to detect SARS-CoV-2 sequences in (D) RNAs extracted from the NP fluid (NPF) and (E) saliva samples of COVID-19 positive patients. The reactions were incubated at 65 °C for 30 min. In (D) and (E) the Cts (N gene) obtained for the paired NP samples are indicated. NTC no template control, HD healthy donor. Figures are representative of three independent experiments.