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Components of the transfection mixture
| Component | Amount per well |
|---|---|
| Reporter pISRE-F-Luc or pGAS-F-Luc | 100 ng 100 ng |
| Internal control construct (e.g., pGAPDH-R-Luc) | 20 ng |
| Viral protein (in pLVX1) | 250 ng |
| Total: | 370 ng |
Fill up each mix to the same amount of total DNA with e.g., empty vector.
